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Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical

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《医学前沿（英文）》 2016年 第10卷 第4期   页码 517-521 doi: 10.1007/s11684-016-0480-9

摘要：

This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients (70.41%). The susceptibility to triple antibiotic ointment (neomycin/bacitracin/polymyxin B) and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.

关键词： antimicrobial susceptibility/resistance     Propionibacterium acnes     topical antibiotics     in vitro study    

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of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

《环境科学与工程前沿（英文）》 2015年 第9卷 第4期   页码 625-633 doi: 10.1007/s11783-014-0711-8

摘要： A novel hybrid material, Cu-PAA/MWCNTs (copper nanoparticles deposited multiwalled carbon nanotubes with poly (acrylic acid) as dispersant, was prepared and expected to obtain a more effective and well-dispersed disinfection material for water treatment. X-ray energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), the X-ray fluorescence (XRF), X-ray photoelectron spectra (XPS), Fourier transform infrared spectra (FT-IR), Raman spectroscopy, and thermal gravimetric analyzer (TGA) were used to characterize the Cu-PAA/MWCNTs. ( ) was employed as the target bacteria. The cell viability determination and fluorescence imaging results demonstrated that Cu-PAA/MWCNTs possessed strong antimicrobial ability on . The deposited Cu was suggested to play an important role in the antimicrobial action of Cu-PAA/MWCNTs.

关键词： multiwalled carbon nanotubes     copper nanoparticles     antimicrobial activity     Escherichia coli (E. coil)    

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吖啶– 核定位序列偶联构建的高活性抗菌肽 Artical

Wei Zhang,Xiaoli Yang,Jingjing Song,Xin Zheng,Jianbo Chen,Panpan Ma,Bangzhi Zhang,Rui Wang

《工程（英文）》 2015年 第1卷 第4期   页码 500-505 doi: 10.15302/J-ENG-2015106

摘要：

多重耐药菌的出现迫切需要发现具有新作用机制的抗生素。在本研究中，笔者将疏水的吖啶分子连接到核定位序列 (NLS) 的N末端合成了一种新型的抗菌药物Acr3-NLS。为了进 一步提高该试剂的抗菌活性，笔者将两个单体Acr3-NLS分子通过二硫键连接合成了二聚体(Acr3-NLS)2。结果显示，与NLS相比，Acr3-NLS，特别是(Acr3-NLS)2，对革兰阴性菌和革兰阳性菌表现出显著的抗菌活性。随后，其作用机制的研究表明，Acr3-NLS 和(Acr3-NLS)2 可通过细胞膜破坏方式和DNA 结合方式杀死细菌。作用于细胞膜和细胞内DNA的双靶点抑杀机制可以降低细菌对Acr3-NLS 和(Acr3-NLS)2 产生耐药性的风险。总之， 本研究提供了一种新的策略，可用于设计具有双重作用机制的高效抗菌药物。

关键词： 吖啶     核定位序列     共轭连接     抗菌活性     作用机制    

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Antimicrobial use in food animal production: situation analysis and contributing factors

Ziping WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 301-311 doi: 10.15302/J-FASE-2018207

摘要：

By measuring the quantity and ways in which antimicrobials are used, and reviewing different technical and socioeconomic factors influencing antimicrobial use at farm level, this study discusses the main knowledge gaps in antimicrobial use in food animal production and provides recommendations for future research and policy development. The review reveals that antimicrobial use in food animals exhibit strong regional and species differences, and there are still large information gaps concerning the current state of antimicrobial use. Factors associated with animal health (including antimicrobial resistance), animal health improvement, economic costs and benefits relevant to animal diseases, and potential technological alternatives or alternative systems all have an impact on antimicrobial use on the farm. There is a clear need to resolve the data gap by monitoring antimicrobial use and developing an analytical framework to better understand farmer behaviors under different technical, economic and environmental circumstances.

关键词： antimicrobial use     drivers for antimicrobial use     food animal production    

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Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

《化学科学与工程前沿（英文）》 2023年 第17卷 第12期   页码 2101-2113 doi: 10.1007/s11705-023-2361-5

摘要： In response to the reduction of food production and economic losses caused by plant bacterial diseases, it is necessary to develop new, efficient, and green pesticides. Natural products are rich and sustainable source for the development of new pesticides due to their low toxicity, easy degradation, and eco-friendliness. In this study, we prepared three series of ursolic acid derivatives and assessed their antibacterial ability. Most target compounds exhibited outstanding antibacterial activities. Among them, the relative optimal EC50 values of Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. citri were 2.23 (A17) and 1.39 (A16) μg·mL–1, respectively. The antimicrobial mechanism showed that compound A17 induced an excessive accumulation and production of reactive oxygen species in bacteria and damaged the cell membrane integrity to kill bacteria. More interestingly, the addition of low concentrations of exogenous hydrogen peroxide enhanced the antibacterial efficacy of compound A17 against Xanthomonas oryzae pv. oryzae. These entertaining results suggested that compound A17 induced an apparent apoptotic behavior in the tested bacteria. Overall, we developed the promising antimicrobial agents that destroyed the redox system of phytopathogenic bacteria, further demonstrating the unprecedented potential of ursolic acid for agricultural applications.

关键词： ursolic acid     antibacterial activities     reactive oxygen species     apoptosis    

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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 203-209 doi: 10.1007/s11705-012-1277-2

摘要： Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

关键词： recombinant destabilase-lysozyme (rec.Dest-Lys)     antimicrobial activity     antifungal activity     medicinal leech     heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)    

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Microwave-assisted synthesis and antimicrobial activities of 2-aryl-3-(naphthalene-1 or 2-yl)-1,3-thiazolidin

Hua CHEN, Yanan LI, Jie BAI, Lian ZHAO, Xiangguo YUAN, Xiaoliu LI, Keqiang CAO

《化学科学与工程前沿（英文）》 2009年 第3卷 第2期   页码 186-191 doi: 10.1007/s11705-009-0004-0

摘要： 2-aryl-3-(naphthalene-1 or 2-yl)-1,3-thiazolidin-4-ones and were synthesized in 41%-67% yield by using microwave-assisted one-pot protocol. The structures of the new compounds , , , , , , and - were confirmed by IR, NMR, MS, and elemental analysis. The antimicrobial activities of the compounds against pv. lachrymans (Smith et Bryan) Young, Dye & Wilkie, Pers., and Blum. were examined. Some of the compounds showed good antifungical activity against Blum.

关键词： microwave-assisted synthesis     naphthyl     thiazolidin-4-one     antimicrobial    

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越南广平和顺化两省猪场分离副猪嗜血杆菌的抗生素耐药表型和耐药基因之间的相关性研究 Article

Chao Nguyen Van, 张丽君, Tam Vu Thi Thanh, Hung Pham Hoang Son, Tuan Tran Ngoc, 黄琦, 周锐

《工程（英文）》 2020年 第6卷 第1期   页码 40-48 doi: 10.1016/j.eng.2019.10.014

摘要：

副猪嗜血杆菌是一种对全球养猪业造成重大经济损失的病原菌。副猪嗜血杆菌防控失败的原因之一是细菌耐药性不断加重。越南拥有亚洲排名第二的生猪产量，但是缺乏针对副猪嗜血杆菌耐药性的相关研究和数据。本研究的目的是调查越南副猪嗜血杆菌的耐药性，并分析耐药性和耐药性基因之间的关系。本研究所使用的副猪嗜血杆菌分离菌株来自我们之前研究中从越南广平省和顺化省猪场分离到的菌株。本研究分别利用肉汤微稀释法和聚合酶链反应（PCR）法检测了这些菌株对25种抗生素的耐药性及相关耐药基因的存在情况。结果显示，这些分离株对甲氧苄啶/磺胺甲恶唑的耐药率达高到94.6%，其次是黏菌素、氯霉素、庆大霉素、青霉素、林可霉素和阿莫西林。这些菌株中相关联耐药基因的检出率分别为blaTEM-1 94.6%、int 76.8%、gyrA 58.9%以及rmtD 50.0%。头孢呋辛、氯霉素和妥布霉素的耐药性表型分别与耐药基因blaROB-1 [优势比（OR） = 26.3, 95% 置信区间（CI）2.7~255.7, p = 0.002]、catl (OR = 25.1, 95% CI 2.4~258.9, p = 0.004)以及strB (OR = 23.5, 95% CI 2.6~212.6, p = 0.001)呈显著正相关。本研究首次揭示了越南中部地区副猪嗜血杆菌的耐药性情况，有助于该疾病的临床控制，能够为降低越南中部的养猪业中该菌的耐药性以及为相关政策制定和临床用药提供指导。

关键词： 副猪嗜血杆菌，抗药性，耐药基因，越南    

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细菌耐药性威胁人类健康 Editorial

沈建忠, Timothy R. Walsh

《工程（英文）》 2022年 第15卷 第8期   页码 1-2 doi: 10.1016/j.eng.2022.06.003

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IDEEA activity monitor: validity of activity recognition for lying, reclining, sitting and standing

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《医学前沿（英文）》 2013年 第7卷 第1期   页码 126-131 doi: 10.1007/s11684-012-0236-0

摘要：

Recent evidence demonstrates the independent negative effects of sedentary behavior on health, but there are few objective measures of sedentary behavior. Most instruments measure physical activity and are not validated as measures of sedentary behavior. The purpose of this study was to evaluate the validity of the IDEEA system’s measures of sedentary and low-intensity physical activities: lying, reclining, sitting and standing. Thirty subjects, 14 men and 16 women, aged 23 to 77 years, body mass index (BMI) between 18 to 34 kg/m2, participated in the study. IDEEA measures were compared to direct observation for 27 activities: 10 lying in bed, 3 lying on a sofa, 1 reclining in a lawn chair, 10 sitting and 3 standing. Two measures are reported, the percentage of activities accurately identified and the percentage of monitored time that was accurately labeled by the IDEEA system for all subjects. A total of 91.6% of all observed activities were accurately identified and 92.4% of the total monitored time was accurately labeled. The IDEEA system did not accurately differentiate between lying and reclining so the two activities were combined for calculating accuracy. Using this approach the IDEEA system accurately identified 96% of sitting activities for a total of 97% of the monitored sitting time, 99% and 99% for standing, 87% and 88% for lying in bed, 87% and 88% for lying on the sofa, and 83% and 83% for reclining on a lawn chair. We conclude that the IDEEA system accurately recognizes sitting and standing positions, but it is less accurate in identifying lying and reclining positions. We recommend combining the lying and reclining activities to improve accuracy. The IDEEA system enables researchers to monitor lying, reclining, sitting and standing with a reasonable level of accuracy and has the potential to advance the science of sedentary behaviors and low-intensity physical activities.

关键词： IDEEA     activity monitor     sedentary behavior    

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Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer

Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA

《化学科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 482-489 doi: 10.1007/s11705-013-1342-5

摘要： In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps: lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions: 50°C, pH 4.0, 2% enzyme/substrate (E/S) ratio, 15 h, and a solid to liquid ratio of 1 ∶ 10. The pectic extract showed a degree of polymerization (DP) profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5–25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen (4-OHT), a classical anticancer drug, which killed 56.5% of the cells.

关键词： pectic extracts     antiproliferative activity     breast cancer     enzymatic treatment    

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Strategies on designing multifunctional surfaces to prevent biofilm formation? ?

Yang Lu,Zhanguo Yue,Wei Wang,Zhiqiang Cao

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 324-335 doi: 10.1007/s11705-015-1529-z

摘要： Bacteria adhesion and biofilm formation have raised severe problems on public health, food industry and many other areas. A variety of reagents and surface coatings have been developed to kill bacteria and/or limit their interaction with surfaces. It has also attracted many efforts to integrate different bactericidal elements together and maximize antibacterial efficiency. Herein, we review mechanisms for both passive and active approaches to resist and kill bacteria respectively, and discuss integrated strategies based on these two approaches. We also offer perspective on future research direction.

关键词： antimicrobial     surface     multifunctional    

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Low intensity ultrasound stimulates biological activity of aerobic activated sludge

LIU Hong, YAN Yixin, WANG Wenyan, YU Yongyong

《环境科学与工程前沿（英文）》 2007年 第1卷 第1期   页码 67-72 doi: 10.1007/s11783-007-0013-5

摘要： This work aims to explore a procedure to improve biological wastewater treatment efficiency using low intensity ultrasound. The aerobic activated sludge from a municipal wastewater treatment plant was used as the experimental material. Oxygen uptake rate (OUR) of the activated sludge (AS) was determined to indicate the changes of AS activity stimulated by ultrasound at 35 kHz for 0 40 min with ultrasonic intensities of 0 1.2 W/cm. The highest OUR was observed at the ultrasonic intensity of 0.3 W/cm and an irradiation period of 10 min; more than 15% increase was achieved immediately after sonication. More significantly, the AS activity stimulated by ultrasound could last 24 h after sonication, and the AS activity achieved its peak value within 8 h after sonication, or nearly 100% higher than the initial level after sonication. Therefore, to improve the wastewater treatment efficiency of bioreactors, ultrasound with an intensity of 0.3 W/cm could be employed to irradiate a part of the AS in the bioreactor for 10 min every 8 h.

关键词： sonication     irradiation     kHz     treatment efficiency     AS activity    

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preparation of Ag@AgCl/Bio-veins composites and their photocatalytic activity and recyclability

《化学科学与工程前沿（英文）》 2023年 第17卷 第7期   页码 906-917 doi: 10.1007/s11705-022-2273-9

摘要： The industrial application of nano-photocatalysts in wastewater treatment has been severely restricted for a long time due to their difficult separation, poor reusability, and low efficiency. In this work, a facile strategy was proposed to enhance the photocatalytic activity and recovery performance of Ag@AgCl nanocatalysts. Biological veins (Bio-veins) with a unique 3D porous construction were used as carriers for the in-situ growth of Ag@AgCl nanoparticles. Scanning electron microscopy results showed that the Ag@AgCl nanoparticles were uniformly loaded on the surface and interior of the Bio-veins, and the size of the Ag@AgCl nanoparticles immobilized on the Bio-veins (50–300 nm) was significantly smaller than Ag@AgCl obtained by the co-precipitation method (1–3 μm). The Bio-veins played a vital role in the photocatalysis reaction system. The degradation efficiency of the Ag@AgCl/Bio-veins(CI4) was up to 3.50 times as high as pure Ag@AgCl. Furthermore, the composites also exhibited excellent recyclability and stability under both visible and solar light. This work provided a suitable strategy for nano-photocatalysts for practical application and may also offer new possibilities for the high-value utilization of biomass materials.

关键词： Ag@AgCl     biological veins     photocatalytic activity     recyclability    

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Effect of 2-butenal manufacture wastewater to methanogenic activity and microbial community

Guangqing Song, Hongbo Xi, Xiumei Sun, Yudong Song, Yuexi Zhou

《环境科学与工程前沿（英文）》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1056-5

摘要：

The inhibition ratio sharply increased with the increasing COD.

The absorbance of UV-vis at 420 nm showed a linear correlation with the SMA.

The molecular structure of EPS has changed when COD was 9585 mg/L.

Illumina Miseq sequencing was employed to reveal the microbial composition.

关键词： 2-butenal manufacture wastewater     Methanogenic activity     Specific methanogenic activity (SMA)     Extracellular polymeric substance (EPS)     Microbial community    

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标题 作者 时间 类型 操作

Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical

null

期刊论文

of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

期刊论文

吖啶– 核定位序列偶联构建的高活性抗菌肽

Wei Zhang,Xiaoli Yang,Jingjing Song,Xin Zheng,Jianbo Chen,Panpan Ma,Bangzhi Zhang,Rui Wang

期刊论文

Antimicrobial use in food animal production: situation analysis and contributing factors

Ziping WU

期刊论文

Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

期刊论文

Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

期刊论文

Microwave-assisted synthesis and antimicrobial activities of 2-aryl-3-(naphthalene-1 or 2-yl)-1,3-thiazolidin

Hua CHEN, Yanan LI, Jie BAI, Lian ZHAO, Xiangguo YUAN, Xiaoliu LI, Keqiang CAO

期刊论文

越南广平和顺化两省猪场分离副猪嗜血杆菌的抗生素耐药表型和耐药基因之间的相关性研究

Chao Nguyen Van, 张丽君, Tam Vu Thi Thanh, Hung Pham Hoang Son, Tuan Tran Ngoc, 黄琦, 周锐

期刊论文

细菌耐药性威胁人类健康

沈建忠, Timothy R. Walsh

期刊论文

IDEEA activity monitor: validity of activity recognition for lying, reclining, sitting and standing

null

期刊论文

Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer

Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA

期刊论文

Strategies on designing multifunctional surfaces to prevent biofilm formation? ?

Yang Lu,Zhanguo Yue,Wei Wang,Zhiqiang Cao

期刊论文

Low intensity ultrasound stimulates biological activity of aerobic activated sludge

LIU Hong, YAN Yixin, WANG Wenyan, YU Yongyong

期刊论文

preparation of Ag@AgCl/Bio-veins composites and their photocatalytic activity and recyclability

期刊论文

Effect of 2-butenal manufacture wastewater to methanogenic activity and microbial community

Guangqing Song, Hongbo Xi, Xiumei Sun, Yudong Song, Yuexi Zhou

期刊论文